Journal: Experimental and Therapeutic Medicine

Article Title: Negative pressure wound therapy enhances bone regeneration compared with conventional therapy in a rabbit radius gap-healing model

doi: 10.3892/etm.2021.9905

Figure Lengend Snippet: VEGF, BMP-2 and OPN protein expression. (A) Representative western blot image and quantification of (B) VEGF, (C) BMP-2 and (D) OPN protein expression at each time point examined in the NPWT and control groups. * P<0.05 and ** P<0.01. VEGF, vascular endothelial growth factor; BMP-2, bone morphogenetic protein 2; OPN, osteopontin; NPWT, negative pressure wound therapy; 2W, week 2; 4W, week 4.

Article Snippet: The membranes were then incubated overnight at 4˚C with TBS-Tween-20 supplemented with one of the following primary antibodies: Mouse anti-β-actin (1:1,000; Boster Biological Technology; cat. no. P60709), mouse anti-VEGF (1:200; Santa Cruz Biotechnology, Inc. cat. no. sc-7269), mouse anti-BMP-2 (1:400; Santa Cruz Biotechnology, Inc. cat. no. sc-137087) or mouse anti-OPN (1:400; Abcam; cat. no. ab228748).

Techniques: Expressing, Western Blot

Journal: European Journal of Histochemistry : EJH

Article Title: Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation

doi: 10.4081/ejh.2017.2772

Figure Lengend Snippet: Histological changes in skeletal muscles on day one after empty vector or BMP-2 gene transfer. a-c) Skeletal muscles on day one after empty vector transfer. a) Haematoxylin-eosin staining showing migration of inflammatory cells (black arrows) and muscle damage (red arrows). b) CD68-positive cells (red arrows) are detected in the spaces between muscle fibres (blue arrows). c) M-cadherin-positive cells (arrows). d-h) Skeletal muscle one day after BMP-2 gene transfer. d) Haematoxylin-eosin staining. e) Magnification of the insert in (d). f ) Human BMP-2 -positive cells (red arrows). g) CD68- positive cells (arrows). h) Pax7-positive cells (arrows). Scale bars: 100 >m.

Article Snippet: Anti-Pax7 rabbit polyclonal, anti-Myod1 mouse monoclonal, antimyogenin mouse monoclonal, anti-M-cadherin goat polyclonal, and anti-BMP-2 mouse monoclonal antibodies (all 1:100; Abcam, Cambridge, UK), and anti-CD68 mouse monoclonal antibody (1:50; Bio- Rad, Hercules, CA, USA) were incubated for 12 h at 4°C.

Techniques: Plasmid Preparation, Staining, Migration

Journal: European Journal of Histochemistry : EJH

Article Title: Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation

doi: 10.4081/ejh.2017.2772

Figure Lengend Snippet: Histological changes in skeletal muscles on day three after empty vector or BMP-2 gene transfer. a,b) Skeletal muscles on day three after empty vector transfer. a) Haematoxylin-eosin staining showing enlarged muscle fibres filled with lymphocyte-like cells (arrows). b) CD68-positive cells (arrows). c-f ) Histological changes on day three after BMP-2 gene transfer. c) Haematoxylin-eosin staining showing enlarged spaces between fibres (arrow). d) CD68-positive cells. e) BMP-2-positive cells are detected only in less damaged muscles (arrows). f ) Myod1-positive cells localised around the spaces between muscle fibres (arrows). Scale bars: 100 >m.

Article Snippet: Anti-Pax7 rabbit polyclonal, anti-Myod1 mouse monoclonal, antimyogenin mouse monoclonal, anti-M-cadherin goat polyclonal, and anti-BMP-2 mouse monoclonal antibodies (all 1:100; Abcam, Cambridge, UK), and anti-CD68 mouse monoclonal antibody (1:50; Bio- Rad, Hercules, CA, USA) were incubated for 12 h at 4°C.

Techniques: Plasmid Preparation, Staining

Journal: European Journal of Histochemistry : EJH

Article Title: Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation

doi: 10.4081/ejh.2017.2772

Figure Lengend Snippet: Histological changes on day five after empty vector or BMP-2 gene transfer. a-e) Skeletal muscles on day five after empty vector transfer. a) Haematoxylin-eosin staining showing spindle-shaped and cytoplasm-enriched cells in the spaces between muscle fibres (arrows). b) CD68-positive cells (arrow). c) Pax7-positive cells (arrow). d) Myod1- positive cells (arrow). e) Myogenin-positive cells are detected around the cell migration area (arrows). f-k) Skeletal muscles on day five after BMP-2 gene transfer. f ) Haematoxylin-eosin staining showing typical muscle fibres. g) CD68-positive cells. h) BMP-2-positive cells are detected in the haematoxylin-positive population, but not in muscle fibres. i) Pax7-positive cells (arrow). j) M-cadherin-positive cells (arrows). k) Myogenin-positive cells (arrows) are detected between muscle fibres. Scale bars: a,b) 100 >m; c-k) 50 >m.

Article Snippet: Anti-Pax7 rabbit polyclonal, anti-Myod1 mouse monoclonal, antimyogenin mouse monoclonal, anti-M-cadherin goat polyclonal, and anti-BMP-2 mouse monoclonal antibodies (all 1:100; Abcam, Cambridge, UK), and anti-CD68 mouse monoclonal antibody (1:50; Bio- Rad, Hercules, CA, USA) were incubated for 12 h at 4°C.

Techniques: Plasmid Preparation, Staining, Migration

Journal: European Journal of Histochemistry : EJH

Article Title: Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation

doi: 10.4081/ejh.2017.2772

Figure Lengend Snippet: Histological changes on day seven after empty vector or BMP-2 gene transfer. a-c) Skeletal muscles on day seven after empty vector transfer. a) Haematoxylin-eosin staining. b) No CD68-positive cells are detected. c) Pax7-positive cells localised around normal muscle fibres (arrow). d-g) Skeletal muscles on day seven after BMP-2 gene transfer. d) Haematoxylin-eosin staining showing migrating spindle-shaped cells (arrows). e) CD68-positive cells (arrows). f ) Pax7-positive cells localised to the spindle-shaped cells migration area (arrows). g) Alkaline phosphatase-positive cells localised to the spindleshaped cell population (arrows). Scale bars: 100 >m.

Article Snippet: Anti-Pax7 rabbit polyclonal, anti-Myod1 mouse monoclonal, antimyogenin mouse monoclonal, anti-M-cadherin goat polyclonal, and anti-BMP-2 mouse monoclonal antibodies (all 1:100; Abcam, Cambridge, UK), and anti-CD68 mouse monoclonal antibody (1:50; Bio- Rad, Hercules, CA, USA) were incubated for 12 h at 4°C.

Techniques: Plasmid Preparation, Staining, Migration